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摘要: |
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本文报道黄金香柳的组织培养和快速繁殖试验。结果表明：以黄金香柳嫩茎作外植体进行培养，不定芽诱导率高，经连续3次培养不定芽增殖倍数可达7.9-10.7。适宜黄金香柳丛芽快速增殖的培养基配方为MS＋6-BA0.5mg/L＋NAA0.01mg/L＋蔗糖30g/L，一次继代培养时，平均每瓶无菌材料可提供45.5个枝芽（≥1cm）用于转接生根。诱导植株生根的最佳培养基配方为MS＋IBA1.0～1.5mg/L＋蔗糖15g/L，适宜的培养时间为21～25d，生根率达96%以上。移植的试管植株90%以上存活。
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关键词:
黄金香柳 组织培养 快速繁殖
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DOI：
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分类号：
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基金项目：广州市林业局项目。 |
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RAPID PROPAGATION OF Melaleuca bracteata F. Fuell. VIA TISSUE CULTURE |
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Abstract: |
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Abstract This paper deals with the preliminary results on tissue culture and rapid propagation of Melaleuca bracteata F. Fuell. . After 3 subcultures, a large number of adventitious buds could be induced from shoot explants on MS and DCR media supplemented with 6-BA0.5～2.0mg/L and NAA0.01mg/L, on which increased the multiple buds nearly tenfold. Nevertheless, the optimum propagation medium for subculture selected from the experiments was MS containing 6-BA0.5mg/L and NAA0.01mg/L, under this procedure be able to obtain 45.5 shoots for rooting culture from one culture bottle in a period of subculture averagely. The trial also identified that the optimum medium for root induction was MS supplemented with IBA 1.0～1.5mg/L and 96% of rooting percentage was obtained. The plantlet survival rate could exceed 90% provided that the nursery condition was acceptable.
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Key words:
Melaleuca bracteata F. Fuell., tissue culture, rapid propagation
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