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The adventitious shoots of limber pine were taken as objects to investigate their induction of adventitious roots. It was found that IBA had no significant effect on the induction of adventitious roots, when IBA and NAA were used together, the abnormal roots were formed. NAA had a better effect on the elongation of adventitious shoots, and the height reached 3.12 cm when NAA was at the concentration of 0.05 mg•L-1, it was significantly different from other concentrations. Different concentration of NAA and different induction time hadn’t improved the frequency of rooting, conversely, helped the formation of callus. Generally, 1/2GD medium, without growth regulator, had a better effect on the induction of adventitious roots, reached 16.67%. It indicated that IBA and NAA was not the key factor that influenced the formation of roots of limber pine.
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