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摘要: |
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开展杉木优良无性系组织培养技术研究在生产上具有重要意义，本文开展了外植体清毒、无菌材料增殖试验、无菌材料生根试验等方面的组织培养研究，繁殖了一批苗木。从试验来看，杉木不同无性系在同一培养基上增殖表现差异显著；杉木优良无性系I不定芽增殖比较好的配方是0.5MS +6BA0.2mg/l +NAA0.2mg/l，生根诱导较好的配方是GGR60.4mg/l+根太阳1.2mg/l；无性系D 比较好的生根诱导配方是IBA0.4mg/l+根太阳1.4mg/l。
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关键词:
杉木 无性系 组织培养
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DOI：
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基金项目：广东省自然科学基金“杉木优良无性系组培快繁技术研究”（5004951）、广东省林业种苗专项“杉木良种选育”、广东省林业科技创新专项“松杉良种选育和高效栽培技术研究与示范”（2008KJCX00501、2009KJCX0071）。 |
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Initial Tissue Culture Research reports forSuperior Clones of Cunninghamia Lanceolata |
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Abstract: |
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Developing tissue culture of Cunninghamia Lanceolata superior clones has great significance for forest wood production. This article carried out some research on explant sterilization, sterile material proliferation and sterile materials rooting of Cunninghamia Lanceolata superior clones, some seedlings have been bred. Judging from the pilot, Different clones of Chinese fir show different proliferation performance in the same medium. For superior clone I, A better formula is 0.5MS +6BA0.2mg/l +NAA0.2mg/l on Proliferation of adventitious buds, and GGR60.4mg/l+ sunroot1.2mg/l on induced Rooting. For superior clone D, better induced root formula is IBA60.4mg/l + sunroot 1.4mg/l.
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Key words:
Cunninghamia Lanceolata clone Tissue culture
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