2020年第5期
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3种霉菌在油茶饼粕固态发酵生长对比

张晖1, 王炳新2, 董斌3, 黄永芳4
1.仲恺农业工程学院;2.广东省台山市林业局;3.广东农工商职业技术学院;4.华南农业大学
摘要:
试验通过对比鲁氏毛霉Mucor roxianus、哈茨木霉Trichoderma harzianum 和黑曲霉Aspergillus niger 3 种菌在未脱茶皂素与已脱茶皂素两种油茶饼粕中的接种比较,观察3 种霉菌在不同温度(20、30、40 ℃)和油茶饼粕含水量(40%、50%、60%、70%)下的生长状况。经过30 天的观察,发现3 种霉菌在未脱茶皂素油茶饼粕中起始生长较慢,其菌丝未能完全遍布到油茶饼粕,在含水量为60% 和70% 油茶饼粕中会出现黄色油状层;然而,3 种霉菌在已脱茶皂素油茶饼粕中生长较快,含水量40%、50% 和60% 处理中菌丝均能布满在茶粕中。结论认为油茶饼粕在作微生物发酵处理时应通过前处理将大部分茶皂素从油茶饼粕中除去,并且油茶饼粕含水量控制在不高于60%,温度适宜控制在30 ℃。
关键词:   油茶;鲁氏毛霉;哈茨木霉;黑曲霉;发酵
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基金项目:广东省重点领域研发计划项目( 2020B020215003),广东省教育厅重点科研项目(2020GCZX009)
 
Comparing growth of three fungi in oil-tea cake fermentation
zhanghui1, Wang Bing Xin2, Dong Bin3, Huang Yong Fang4
1.Zhong Kai Nong Ye Gong Cheng Xue Yuan;2.Guangdong Taishan city forestry bureau;3.Guangdong AIB Polytechnic;4.South China Agricultural University
Abstract:
By comparing the inoculation of Trichoderma harzianum, Mucor roxianus and Aspergillus niger in oil-tea cake, the growth of three fungi at different temperatures (20, 30, 40 ℃ ) and the water content of oil-tea cake (40%, 50%, 60%, 70%). After 30 days’ observation, it was found that the three fungi started to grow slowly in the oil-tea cake(with saponin), and their hyphae did not completely spread to the oil-tea cake. While the fungi grew rapidly in the non-saponin oil-tea cake , and the hyphae could be filled in the oil-tea cake with 40%, 50% and 60% water content. The results suggested that saponin should be removed from oil-tea cake by pretreatment, and the water content of oil tea cake should be controlled less than 60% and the temperature should be controlled at 30℃ .
Key words:   Camellia oleifera; Mucor roxianus; Trichoderma harzianum; Aspergillus niger; Fermentation